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Cat.No. : CSC-RT0108
Host Cell: 293T Target Gene: POLR3B
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0108 |
| Description | 293T-POLR3B cell line is a stable cell line with a homozygous knockout of POLR3B |
| Target Gene | POLR3B |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
POLR3B Knockout Cell Line-293T has truly been a distinguished helper in my research. Its outstanding knockout efficiency and consistent results have allowed me to unravel the functional significance of specific genes with remarkable precision.
United States
05/12/2021
I am truely impressed with the performance of POLR3B Knockout Cell Line-293T. Its reliable and reproducible knockout outcomes have significantly advanced my understanding of gene function and its implications in disease.
United States
08/15/2021
Using POLR3B Knockout Cell Line-293T has been a seamless experience. Its user-friendly interface, combined with its exceptional knockout efficiency, has made gene editing experiments more streamlined and successful.
Germany
10/09/2021
I cannot emphasize enough how invaluable POLR3B Knockout Cell Line-293T has been for my studies. Its high success rate and consistent knockout outcomes have provided me with a solid foundation for in-depth functional investigations.
United States
01/27/2022
POLR3B Knockout Cell Line-293T is a must-have for any researcher seeking to unravel the complexities of gene function. Its outstanding performance, reliability, and ease of use have accelerated my research progress and opened up new research avenues.
United States
07/19/2022
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