PCP20

The pCP20 vector is a sophisticated tool in molecular biology, renowned for its antibiotic resistance capabilities and temperature-sensitive replication. This vector came into existence in 1995, thanks to the dedicated efforts of Dr. W. Wackernagel, from the Genetics department, Biological Sciences Faculty, at the University Oldenburg. The plasmid encompasses 9332 base pairs. This plasmid harbors resistance to ampicillin and CmR, and demonstrates shifts in replication tendencies based on temperature levels. Furthermore, it showcases thermal induction of FLP synthesis.

In particular experiments, CmR and KmR mutants underwent transformation with pCP20. This process occurred in the course of resistant transformation selection, performed under a moderate temperature of 30° Celsius. Ampicillin-resistant transforms were the chosen sample, and their further cultivation was achieved during a nonselective, colony-purifying process at 43° Celsius. An in-depth assessment of these transformed samples showed that a majority had lost the FRT-flanked resistance gene and the FLP helper plasmid simultaneously.