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Cat.No. : CSC-RT0144
Host Cell: 293T Target Gene: PAFAH1B3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0144 |
| Description | 293T-PAFAH1B3 (-/-) is a stable cell line with a homozygous knockout of human PAFAH1B3 |
| Background | This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with mental retardation, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009] |
| Target Gene | PAFAH1B3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
PAFAH1B3 Knockout Cell Line-293T has revolutionized my gene editing experiments, providing a reliable and efficient solution for targeted gene modifications.
United States
02/23/2023
I am directly impressed by the high success rate of gene knockouts achieved with PAFAH1B3 Knockout Cell Line-293T, allowing me to confidently investigate the functions of specific genes.
United States
04/11/2023
The researcher-friendly nature of PAFAH1B3 Knockout Cell Line-293T's protocol has made it easy for me to integrate it into my experimental workflow, saving me time and effort.
United Kingdom
05/09/2023
The consistent and reproducible results obtained using PAFAH1B3 Knockout Cell Line-293T have strengthened the reliability of my research outcomes.
United States
05/31/2022
I highly appreciate the exceptional technical support provided by the manufacturer, who has been readily available to address any questions or concerns I had during the gene knockout process.
United States
04/01/2023
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