Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0299
Target Gene : NUCKS1 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0299 |
| Description | 293T-NUCKS1 (-/-) is a stable cell line with a homozygous knockout of human NUCKS1 |
| Background | This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. [provided by RefSeq, Aug 2010] |
| Target Gene | NUCKS1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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NUCKS1 Knockout Cell Line-293T's outstanding efficacy has surpassed my expectations, consistently delivering precise and reliable results in my experiments.
NUCKS1 Knockout Cell Line-293T's user-friendly design and straightforward protocol have made it exceptionally easy to use, saving time and reducing complexities in my research workflow.
NUCKS1 Knockout Cell Line-293T's efficiency in gene editing has been remarkable, enabling me to achieve targeted knockout with a high success rate, boosting the confidence in my experimental outcomes.
Through NUCKS1 Knockout Cell Line-293T's consistent performance, this reagent has become an indispensable tool in my lab, streamlining the process of gene manipulation and expediting the pace of my research.
I am impressed by the reproducibility of results obtained with NUCKS1 Knockout Cell Line-293T, allowing me to validate findings across multiple experiments and strengthening the reliability of my data.
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