Cat.No. : CSC-RT0142
Host Cell: 293T Target Gene: NTPCR
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0142 |
| Description | 293T-NTPCR (-/-) is a stable cell line with a homozygous knockout of human NTPCR |
| Background | The protein encoded by this gene is a non-specific nucleoside triphosphatase that is slow-acting in vitro. This gene is overexpressed in many tumor tissues, and while it is not essential for the cell, overexpression is cytotoxic. However, the cytotoxicity is not related to its triphosphatase activity. [provided by RefSeq, Jul 2016] |
| Target Gene | NTPCR |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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It is a product that relies on relatively advanced gene editing technology, yet it is not too costly.
The gene editing technology used in this product meets almost all the expectations needed for research, so this product saves a lot of time
The technology used in this product is well regarded and the knockout of NTPCR genes is very easy to do.
NTPCR Knockout Cell Line-293T has been a game-changer in my research, enabling me to unravel the intricacies of gene function with precision and confidence.
I am frighteningly impressed by the efficiency and effectiveness of NTPCR Knockout Cell Line-293T, which has consistently delivered successful gene knockouts and facilitated insightful scientific discoveries.
The user-friendly nature of NTPCR Knockout Cell Line-293T's workflow has made it easy for me to integrate it into my experimental pipeline, saving time and effort.
The reliable and reproducible results obtained using NTPCR Knockout Cell Line-293T have reinforced the robustness of my findings and strengthened the overall scientific rigor of my research.
I am so glad for the exceptional quality and performance of NTPCR Knockout Cell Line-293T, which has elevated the standard of my genetic research and expanded the possibilities in my field of study.
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