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Cat.No. : CSC-RT0218
Host Cell: 293T Target Gene: NPM3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0218 |
| Description | 293T-NPM3 (-/-) is a stable cell line with a homozygous knockout of human NPM3 |
| Background | The protein encoded by this gene is related to the nuclear chaperone phosphoproteins, nucleoplasmin and nucleophosmin. This protein is strongly expressed in diverse cell types where it localizes primarily to the nucleus. Based on its similarity to nucleoplasmin and nucleophosmin, this protein likely functions as a molecular chaperone in the cell nucleus. [provided by RefSeq, Oct 2008] |
| Target Gene | NPM3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The impact of NPM3 Knockout Cell Line-293T extends beyond my immediate research, as it has sparked collaborations with other labs interested in exploring similar gene editing applications.
United States
02/23/2023
Not only has NPM3 Knockout Cell Line-293T accelerated my research progress, but it has also helped me secure funding and support for future studies due to the high-quality data it consistently produces.
United States
04/11/2023
The manufacturer's commitment to ongoing product improvement and updates ensures that I have access to the latest advancements in gene knockout technology.
Germany
08/09/2023
I have recommended NPM3 Knockout Cell Line-293T to my peers and colleagues, as I believe it will continue to play a pivotal role in advancing genetic research globally.
United States
08/23/2020
NPM3 Knockout Cell Line-293T has been a reliable and valuable companion throughout my scientific journey, and I eagerly anticipate the future discoveries it will enable.
United States
05/12/2021
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