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Cat.No. : CSC-RT0114
Host Cell: SCC-9 Target Gene: NGFR
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0114 |
| Description | SCC-9-NGFR cell line is a stable cell line with a homozygous knockout of NGFR |
| Target Gene | NGFR |
| Host Cell | SCC-9 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I wholeheartedly endorse NGFR Knockout Cell Line-SCC-9 to my fellow scientists. Its outstanding performance, ease of use, and consistent knockout outcomes have elevated the quality of my research and accelerated my progress towards scientific breakthroughs.
United States
08/15/2021
NGFR Knockout Cell Line-SCC-9 has truly been a Efficient assistant in my research journey. Its exceptional knockout efficiency and consistent results have allowed me to delve deep into the functional role of specific genes, opening up new avenues of scientific exploration.
Germany
10/09/2021
I am very impressed with the performance of NGFR Knockout Cell Line-SCC-9. Its reliable and reproducible knockout outcomes have provided me with a solid foundation for understanding the intricate mechanisms of gene function and regulation.
United States
01/27/2022
Using NGFR Knockout Cell Line-SCC-9 has been a seamless experience. Its user-friendly interface and high knockout efficiency have made the gene editing process more efficient and successful, saving me valuable time and resources.
United States
07/19/2022
I cannot overstate the significance of NGFR Knockout Cell Line-SCC-9 in my studies. Its exceptional performance and consistent knockout results have empowered me to make meaningful contributions to my field and advance our understanding of genetic mechanisms.
France
11/03/2022
I recommend NGFR Knockout Cell Line-SCC-9 to fellow researchers. Its outstanding performance, ease of use, and reliable knockout outcomes have transformed my research, leading to important discoveries and publications.
United States
02/23/2023
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