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Cat.No. : CSC-RT0264
Host Cell: 293T Target Gene: NDUFA11
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0264 |
| Description | 293T-NDUFA11 (-/-) is a stable cell line with a homozygous knockout of human NDUFA11 |
| Background | This gene encodes a subunit of the membrane-bound mitochondrial complex I. Complex I is composed of numerous subunits and functions as the NADH-ubiquinol reductase of the mitochondrial electron transport chain. Mutations in this gene are associated with severe mitochondrial complex I deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010] |
| Target Gene | NDUFA11 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
As I collaborate with clinicians, the practical utility of NDUFA11 Knockout Cell Line-293T becomes evident in its potential to shed light on the genetic underpinnings of diseases, facilitating the development of personalized treatment approaches.
United States
08/23/2020
In the realm of environmental research, NDUFA11 Knockout Cell Line-293T's ability to modify genes in model organisms offers a powerful tool for studying the impact of specific genes on ecological processes and ecosystem dynamics.
United States
05/12/2021
The simplicity of using NDUFA11 Knockout Cell Line-293T has allowed researchers with varying levels of expertise to employ gene editing techniques effectively, democratizing access to genetic research tools.
United States
08/15/2021
As I present my findings at conferences and engage with stakeholders, the versatility and reliability of NDUFA11 Knockout Cell Line-293T garner appreciation from the scientific community and potential collaborators alike.
United States
10/09/2021
The successful integration of NDUFA11 Knockout Cell Line-293T into my research protocols has fostered a culture of innovation in my lab, encouraging my team to explore novel research questions and push the boundaries of genetic understanding.
United States
01/27/2022
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