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Cat.No. : CSC-RT0286
Host Cell: 293T Target Gene: NAP1L1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0286 |
| Description | 293T-NAP1L1 (-/-) is a stable cell line with a homozygous knockout of human NAP1L1 |
| Background | This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms; however, not all have been fully described. [provided by RefSeq, Apr 2015] |
| Target Gene | NAP1L1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
In the realm of scientific discovery, NAP1L1 Knockout Cell Line-293T has become an integral part of my toolkit, a reliable companion that emboldens me to embrace complexity and seek answers to some of nature's most intriguing questions.
Germany
05/12/2021
As I collaborate with colleagues from different disciplines, I am continually reminded of the interdisciplinary potential of NAP1L1 Knockout Cell Line-293T, bridging the gaps between genetics, medicine, agriculture, and beyond.
United States
08/15/2021
The transformative impact of NAP1L1 Knockout Cell Line-293T reaches far beyond my lab bench, as its contributions reverberate through scientific literature and inspire future generations of researchers.
United States
10/09/2021
The precision and efficiency of NAP1L1 Knockout Cell Line-293T have not only accelerated my research but also elevated my appreciation for the beauty and sophistication of genetic processes that shape all living organisms.
France
01/27/2022
the exceptional customer support I have received has exceeded my expectations, demonstrating the company's commitment to customer satisfaction.
United States
07/19/2022
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