Mouse Sparc (His) HEK293 Cell Lysate

Name: Mouse Sparc (His) HEK293 Cell Lysate
SKU: CLOE-2660
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2660 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2660
Introduction	This cell lysate overexpresses mouse Sparc with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified.
Target Gene	Sparc
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus ]
Gene Symbol	Sparc
Synonyms	BM-40; AA517111
Gene Description	secreted acidic cysteine rich glycoprotein
Gene ID	20692
Uni Prot ID	P07214
m RNA Refseq	NM_009242.4
Protein Refseq	NP_033268.1
Chromosome Location	11 B1; 11 29.9 cM
Function	calcium ion binding; extracellular matrix binding;
Pathway	Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Senescence and Autophagy, organism-specific biosystem; TGF-beta Receptor Signaling Pathway, organism-specific biosystem;

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