Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2716 Host Cell : HEK293
| Cat. No. | CLOE-2716 |
| Introduction | This cell lysate overexpresses mouse Pecam1 (Met1-Lys590) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Pecam1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Pecam1 platelet/endothelial cell adhesion molecule 1 [ Mus musculus ] |
| Gene Symbol | Pecam1 |
| Synonyms | Cd31; Pecam; C85791; PECAM-1 |
| Gene Description | platelet/endothelial cell adhesion molecule 1 |
| Gene ID | 18613 |
| Uni Prot ID | Q08481 |
| m RNA Refseq | NM_001032378.1 |
| Protein Refseq | NP_001027550.1 |
| Chromosome Location | 11 E1; 11 |
| Function | protein binding; |
| Pathway | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; Malaria, organism-specific biosystem; Malaria, conserved biosystem; |
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