Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2686 Host Cell : HEK293
| Cat. No. | CLOE-2686 |
| Introduction | This cell lysate overexpresses mouse Pdgfc (Val235-Gly345) with the Fc region of human IgG1 at the N-terminus. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Pdgfc |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Pdgfc platelet-derived growth factor, C polypeptide [ Mus musculus ] |
| Gene Symbol | Pdgfc |
| Synonyms | PDGF-C; AI647969; 1110064L01Rik |
| Gene Description | platelet-derived growth factor, C polypeptide |
| Gene ID | 54635 |
| Uni Prot ID | Q8CI19 |
| m RNA Refseq | NM_019971.2 |
| Protein Refseq | NP_064355.1 |
| Chromosome Location | 3 E3; 3 |
| Function | cell surface binding; growth factor activity; platelet-derived growth factor receptor binding; platelet-derived growth factor receptor binding; protein homodimerization activity; |
| Pathway | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; |
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