Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2825 Host Cell : HEK293
| Cat. No. | CLOE-2825 |
| Introduction | This cell lysate overexpresses mouse Mme (Tyr52-Trp750) with a N-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Mme |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Mme membrane metallo endopeptidase [ Mus musculus ] |
| Gene Symbol | Mme |
| Synonyms | NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik |
| Gene Description | membrane metallo endopeptidase |
| GeneID | 17380 |
| Uni ProtID | Q61391 |
| mRNA Refseq | NM_008604.3 |
| Protein Refseq | NP_032630.2 |
| Chromosome Location | 3 E1; 3 29.6 cM |
| Function | endopeptidase activity; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; peptide binding; zinc ion binding; |
| Pathway | Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Metabolism of Angiotensinogen to Angiotensins, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; |
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