Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2710 Host Cell : HEK293
| Cat. No. | CLOE-2710 |
| Introduction | This cell lysate overexpresses mouse Fgf21 with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Fgf21 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Fgf21 fibroblast growth factor 21 [ Mus musculus ] |
| Gene Symbol | Fgf21 |
| Gene Description | fibroblast growth factor 21 |
| Gene ID | 56636 |
| Uni Prot ID | Q9JJN1 |
| m RNA Refseq | NM_020013.4 |
| Protein Refseq | NP_064397.1 |
| Chromosome Location | 7; 7 B2 |
| Function | fibroblast growth factor receptor binding; growth factor activity; |
| Pathway | ESC Pluripotency Pathways, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Melanoma, organism-specific biosystem; Melanoma, conserved biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, conserved biosystem; |
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