Mouse F9 (His) HEK293 Cell Lysate

Name: Mouse F9 (His) HEK293 Cell Lysate
SKU: CLOE-2743
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2743 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2743
Introduction	This cell lysate overexpresses mouse F9 with a C-terminal polyhistidine tag and a signal peptide at the N-terminus. Purification of target protein from this cell lysate has been verified.
Target Gene	F9
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	F9 coagulation factor IX [ Mus musculus ]
Gene Symbol	F9
Synonyms	Cf9; Cf-9; AW111646
GeneID	14071
Uni ProtID	A2AEU0
mRNA Refseq	NM_007979.1
Protein Refseq	NP_032005.1
Chromosome Location	X A6-A7; X 22.0 cM
Function	calcium ion binding; catalytic activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity;
Pathway	Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Gamma-carboxylation of protein precursors, organism-specific biosystem; Gamma-carboxylation, transport, and amino-terminal cleavage of proteins, organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; PTM: gamma carboxylation, hypusine formation and arylsulfatase activation, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; Removal of aminoterminal propeptides from gamma-carboxylated proteins, organism-specific biosystem; Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus, organism-specific biosystem;

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