Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2612 Host Cell : HEK293
| Cat. No. | CLOE-2612 |
| Introduction | This cell lysate overexpresses mouse Epha4 (Met1-Thr547) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Epha4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Epha4 Eph receptor A4 [ Mus musculus ] |
| Gene Symbol | Epha4 |
| Synonyms | rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik |
| Gene Description | Eph receptor A4 |
| Gene ID | 13838 |
| Uni Prot ID | Q03137 |
| m RNA Refseq | NM_007936.3 |
| Protein Refseq | NP_031962.2 |
| Chromosome Location | 1 C1-C5; 1 43.0 cM |
| Function | ATP binding; DH domain binding; GPI-linked ephrin receptor activity; PH domain binding; ephrin receptor activity; ephrin receptor binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein tyrosine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity; transmembrane-ephrin receptor activity; |
| Pathway | Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; |
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