Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2919 Host Cell : HEK293
| Cat. No. | CLOE-2919 |
| Introduction | This cell lysate overexpresses mouse Ctsz (NP_071720.1) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Ctsz |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Ctsz cathepsin Z [ Mus musculus ] |
| Gene Symbol | Ctsz |
| Synonyms | CTSX; AI787083; AU019819; D2Wsu143e |
| Gene Description | cathepsin Z |
| GeneID | 64138 |
| Uni ProtID | Q545I6 |
| mRNA Refseq | NM_022325.4 |
| Protein Refseq | NP_071720.1 |
| Chromosome Location | 2 H4; 2 103.5 cM |
| Function | cysteine-type peptidase activity; hydrolase activity; peptidase activity; |
| Pathway | Clathrin derived vesicle budding, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Lysosome Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; Metabolism of Angiotensinogen to Angiotensins, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; |
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