Mouse Car14 (His) HEK293 Cell Lysate

Name: Mouse Car14 (His) HEK293 Cell Lysate
SKU: CLOE-2991
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2991 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2991
Introduction	This cell lysate overexpresses mouse Car14 (Met1-Met290) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified.
Target Gene	Car14
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	Car14 carbonic anhydrase 14 [ Mus musculus ]
Gene Symbol	Car14
Synonyms	Ca14; AW536446
Gene Description	carbonic anhydrase 14
Gene ID	23831
Uni Prot ID	Q9WVT6
m RNA Refseq	NM_011797.2
Protein Refseq	NP_035927.1
Chromosome Location	3 F2.1; 3
Function	carbonate dehydratase activity; lyase activity; metal ion binding;
Pathway	Metabolism, organism-specific biosystem; Reversible Hydration of Carbon Dioxide, organism-specific biosystem;

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