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Mouse Asah2 (His) HEK293 Cell Lysate

For research use only. Not intended for any clinical use.

Cat. No. :   CLOE-2994 Host Cell :   HEK293

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Cell Lysate Information

Gene Information

Cat. No. CLOE-2994
Introduction This cell lysate overexpresses mouse Asah2 (Thr34-Thr756) with a N-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified.
Target Gene Asah2
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications Positive control for Western Blot (WB), antibody verification, protein purification.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Storage Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping Ice package
Gene Name Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus ]
Gene Symbol Asah2
Synonyms AI585898
Gene Description N-acylsphingosine amidohydrolase 2
Gene ID 54447
Uni Prot ID B9EHG7
m RNA Refseq NM_018830.1
Protein Refseq NP_061300.1
Chromosome Location 19; 19 C3
Function ceramidase activity; hydrolase activity;
Pathway Glycosphingolipid metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phospholipid metabolism, organism-specific biosystem; Signal Transduction of S1P Receptor, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem;
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