Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2996 Host Cell : HEK293
| Cat. No. | CLOE-2996 |
| Introduction | This cell lysate overexpresses mouse Acy1 with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Acy1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Acy1 aminoacylase 1 [ Mus musculus ] |
| Gene Symbol | Acy1 |
| Synonyms | Acy-1; 1110014J22Rik |
| Gene Description | aminoacylase 1 |
| Gene ID | 109652 |
| Uni Prot ID | Q99JW2 |
| m RNA Refseq | NM_025371.2 |
| Protein Refseq | NP_079647.1 |
| Chromosome Location | 9 F1; 9 60.0 cM |
| Function | aminoacylase activity; hydrolase activity; metal ion binding; metallopeptidase activity; |
| Pathway | Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Urea cycle and metabolism of amino groups, organism-specific biosystem; |
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