Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2792 Host Cell : HEK293
| Cat. No. | CLOE-2792 |
| Introduction | This cell lysate overexpresses mouse Acp5 with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Acp5 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Acp5 acid phosphatase 5, tartrate resistant [ Mus musculus ] |
| Gene Symbol | Acp5 |
| Synonyms | TRAP; TRACP |
| Gene Description | acid phosphatase 5, tartrate resistant |
| Gene ID | 11433 |
| Uni Prot ID | Q05117 |
| m RNA Refseq | NM_007388.3 |
| Protein Refseq | NP_031414.1 |
| Chromosome Location | 9 A3; 9 6.0 cM |
| Function | acid phosphatase activity; acid phosphatase activity; acid phosphatase activity; ferric iron binding; ferrous iron binding; hydrolase activity; metal ion binding; |
| Pathway | Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; NAD phosphorylation and dephosphorylation, organism-specific biosystem; NAD salvage pathway II, organism-specific biosystem; |
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