Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2633 Host Cell : HEK293
| Cat. No. | CLOE-2633 |
| Introduction | This cell lysate overexpresses mouse Ache (NP_033729.1) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Ache |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Ache acetylcholinesterase [ Mus musculus ] |
| Gene Symbol | Ache |
| Synonyms | mE1a; mE1b; mE1c; mE1d; mE1e; mE1d; mE1c-long |
| Gene Description | acetylcholinesterase |
| Gene ID | 11423 |
| Uni Prot ID | P21836 |
| m RNA Refseq | NM_009599.3 |
| Protein Refseq | NP_033729.1 |
| Chromosome Location | 5 G2; 5 77.0 cM |
| Function | acetylcholine binding; acetylcholinesterase activity; acetylcholinesterase activity; carboxylesterase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; laminin binding; protein homodimerization activity; protein self-association; serine hydrolase activity; |
| Pathway | Acetylcholine Synthesis, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Glycerophospholipid biosynthesis, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; |
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