Pages
Products
LRRC59 Knockout Cell Line-293T

LRRC59 Knockout Cell Line-293T

Cat.No. :  CSC-RT0307

Host Cell:  293T Target Gene:  LRRC59

Size:  1x10^6 cells/vial, 1mL Validation:  Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0307
Description 293T-LRRC59 (-/-) is a stable cell line with a homozygous knockout of human LRRC59
Target Gene LRRC59
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
Quick Inquiry

Publications

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Adaptability

LRRC59 Knockout Cell Line-293T's adaptability supports a wide range of research objectives, fueling innovation.

United States

01/27/2022

Precision

LRRC59 Knockout Cell Line-293T's reliability and precision make it a cornerstone of my experiments.

Germany

07/19/2022

Invaluable

In the pursuit of genetic understanding, LRRC59 Knockout Cell Line-293T is an invaluable tool.

United States

11/03/2022

Effectiveness

With each knockout achieved, LRRC59 Knockout Cell Line-293T affirms its reliability and effectiveness.

United States

02/23/2023

Impact

LRRC59 Knockout Cell Line-293T Versatility in applications ensures broad-reaching impact across various fields.

France

04/11/2023

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER