Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0166
Target Gene : LMAN1 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0166 |
| Description | 293T-LMAN1 (-/-) is a stable cell line with a homozygous knockout of human LMAN1 |
| Background | The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015] |
| Target Gene | LMAN1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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LMAN1 Knockout Cell Line-293T has been a game-changer in my research, providing a reliable and efficient method for precise gene editing and unlocking new insights into gene function.
I am impressed by the high success rate of gene knockouts achieved with LMAN1 Knockout Cell Line-293T, allowing me to study the roles of specific genes with accuracy and confidence.
The clear instructions provided with LMAN1 Knockout Cell Line-293T have made it easy to incorporate into my experimental workflow, saving time and ensuring consistent results.
The consistent and reproducible outcomes obtained using LMAN1 Knockout Cell Line-293T have strengthened the scientific validity of my research findings and supported the development of new hypotheses.
I am thankful for the exceptional customer support provided by the manufacturer, who has been responsive and knowledgeable, providing valuable assistance throughout my gene knockout experiments.
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