KDELR2 Knockout Cell Line-293T

Name: KDELR2 Knockout Cell Line-293T
SKU: CSC-RT0275
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0275

Target Gene : KDELR2 Host Cell : 293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT0275
Description	293T-KDELR2 (-/-) is a stable cell line with a homozygous knockout of human KDELR2
Target Gene	KDELR2
Host Cell	293T
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Q & A

Customer Reviews

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83\% identical to the KDELR1 gene product. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Simplifies

KDELR2 Knockout Cell Line-293T simplifies intricate genetic manipulations, fostering impactful discoveries.

United States

2021-10-09

Ease of use

KDELR2 Knockout Cell Line-293T's reliability and ease of use amplify my ability to explore gene functions comprehensively.

France

2022-01-27

Excellent performance

Across experiments, KDELR2 Knockout Cell Line-293T's consistent performance bolsters the credibility of my findings.

United States

2022-07-19

Accelerates

KDELR2 Knockout Cell Line-293T's practicality accelerates scientific progress, from basic research to potential applications.

United States

2022-11-03

Effectiveness

With KDELR2 Knockout Cell Line-293T's unwavering effectiveness, this reagent is an essential tool for pushing genetic boundaries.

United States

2023-02-23

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KDELR2 Knockout Cell Line-293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Simplifies

Ease of use

Excellent performance

Accelerates

Effectiveness

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