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Cat.No. : CSC-RT0087
Host Cell: HepG2 Target Gene: KAT7
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0087 |
| Description | HepG2-KAT7 cell line is a stable cell line with a homozygous knockout of KAT7 |
| Target Gene | KAT7 |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I was skeptical about using a gene knockout reagent when the experiment was first conducted, but KAT7 Knockout Cell Line-HepG2 has proven to be very effective. It is easy to use, and the results are consistent and reliable.
United Kingdom
10/09/2021
KAT7 Knockout Cell Line-HepG2 has made gene editing much more accessible and efficient for me. It is a valuable tool for any researcher looking to edit genes efficiently and reliably.
United States
01/27/2022
KAT7 Knockout Cell Line-HepG2 is a real good helper. It is user-friendly, efficient, and consistently produces high-quality results. I couldn't ask for more.
United States
07/19/2022
I have been using KAT7 Knockout Cell Line-HepG2 for a few weeks now, and I am already impressed with the results. It is incredibly efficient, and the knockout efficiency is very high.
United States
11/03/2022
KAT7 Knockout Cell Line-HepG2 has simplified the gene editing process for me. It is easy to use, and the results are always consistent and reliable. I highly recommend it to any researcher.
United States
02/23/2023
KAT7 Knockout Cell Line-HepG2 is simply amazing. It has exceeded my expectations in terms of efficiency and reliability. I will definitely be using it in all my future experiments.
United States
04/11/2023
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