Human YBX1 Knockout Cell Line-HeLa

Name: Human YBX1 Knockout Cell Line-HeLa
SKU: CSC-RT0553
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0553

Target Gene : YBX1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0553
Description	A stable cell line with a homozygous knockout of human YBX1 using CRISPR/Cas9.
Target Gene	YBX1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	YBX1
GeneID	4904

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Purity

The Human YBX1 Knockout Cell Line-HeLa is characterized by its high purity and consistency. The cells are carefully isolated and cultured to ensure a homogenous population, minimizing variations in experimental results.

United States

2020-08-23

Functionalality

The Human YBX1 Knockout Cell Line-HeLa retains functionality, enabling researchers to study the consequences of YBX1 gene knockout on cellular processes and pathways. The cells can be used in various experimental setups to assess the impact of YBX1 deficiency on gene expression, protein function, and cell behavior.

United States

2021-05-12

Infectivity and safety

The Human YBX1 Knockout Cell Line-HeLa is carefully monitored for infectivity and safety. The cells are tested for the absence of contaminants and are guaranteed to be free from adventitious agents, ensuring safe handling and use in the laboratory.

United States

2021-08-15

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Human YBX1 Knockout Cell Line-HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Purity

Functionalality

Infectivity and safety

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