Human THPO(His) HEK293 Cell Lysate

Name: Human THPO(His) HEK293 Cell Lysate
SKU: CLOE-1457
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1457 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1457
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human THPO (NP_000451.1) (Met1-Leu192). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	THPO
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	THPO thrombopoietin [ Homo sapiens ]
Gene Symbol	THPO
Synonyms	ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
Gene Description	thrombopoietin (myeloproliferative leukemia virus oncogene ligand, megakaryocyte growth and development factor)
Gene ID	7066
Uni Prot ID	P40225
m RNA Refseq	NM_000460.2
Protein Refseq	NP_000451.1
Chromosome Location	3q27
Function	cytokine activity; growth factor activity; hormone activity;
Pathway	Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Hemostasis, organism-specific biosystem; Platelet Aggregation (Plug Formation), organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem;
MIM	600044

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