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Human THAP1 adenoviral particles

Human THAP1 adenoviral particles

Cat.No. :  AD00267Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Gene Informationn

Cat. No. AD00267Z
Target Gene THAP1
Species Human
Product Type Adenoviral particle
Insert THAP1
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
Gene Name
Gene Symbol
Synonyms
Gene Description
Gene ID
UniProt ID
mRNA Refseq
Protein Refseq
Chromosome Location
Function
MIM
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THAP1 (THAP domain containing 1) is a gene encoding a zinc finger transcription factor that plays a key role in regulating endothelial cell proliferation, apoptosis, and cell cycle progression. THAP1 is located on human chromosome 8p11.21 and contains a THAP domain, a conserved DNA binding motif that targets specific promoter regions to regulate gene expression. THAP1 mutations are associated with dystonia 6 (DYT6), a movement disorder characterized by involuntary muscle contractions, highlighting its importance in neurological function. In addition to its association with dystonia, THAP1 is involved in epigenetic regulation, transcriptional repression, and angiogenesis control, making it a gene of interest in basic research and therapeutic development. Human THAP1 adenoviral particles are genetically engineered viral vectors designed to deliver the THAP1 gene to target cells for functional studies or potential gene therapy applications. These particles are based on adenovirus serotype 5 (Ad5) engineered to be replication-defective for improved safety. This adenoviral system provides high transduction efficiency in a variety of cell types, including primary and dividing cells, making it an ideal tool for overexpressing THAP1 in vitro or in vivo. Human THAP1 adenoviral particles are widely used to study the role of THAP1 in transcriptional regulation, neurological diseases, and vascular biology. Its application extends to preclinical models of dystonia and cancer research to explore the regulatory mechanisms of THAP1.
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Customer Reviews
Great Customer Support

Had a few technical questions before ordering, and Creative Biogene’s team responded quickly with detailed guidance. Their expertise made the purchasing decision easy.

French

08/07/2023

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