The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
Base Excision Repair; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway; Cleavage of the damaged pyrimidine; Depyrimidination; Displacement of DNA glycosylase by APE1; Recognition and association of DNA glycosylase with site containing an affected pyrimidine; Resolution of AP sites via the multiple-nucleotide patch replacement pathway;
RNA polymerase II transcription cofactor activity; SUMO binding; damaged DNA binding; mismatched DNA binding; protein binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity