Human SPTLC1 adenoviral particles

Name: Human SPTLC1 adenoviral particles
SKU: AD15490Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AD15490Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	AD15490Z
Description	Adenovirus with ORF of serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 2.
Gene	SPTLC1
Species	Human
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

Gene Name	SPTLC1 serine palmitoyltransferase, long chain base subunit 1 [ Homo sapiens ]
Gene Symbol	SPTLC1
Synonyms	HSAN; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1; hLCB1
Gene Description	serine palmitoyltransferase, long chain base subunit 1
Gene ID	10558
Uni Prot ID	O15269
m RNA Refseq	NM_178324.1
Protein Refseq	NP_847894.1
Chromosome Location	9q22.2
Function	protein binding; pyridoxal phosphate binding; serine C-palmitoyltransferase activity;
Pathway	Ceramide biosynthesis, organism-specific biosystem; Ceramide biosynthesis, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phospholipid metabolism, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem;
MIM	605712

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