Human SFN Knockout Cell Line-HCT116

Name: Human SFN Knockout Cell Line-HCT116
SKU: CSC-RT0040
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0040

Target Gene : SFN Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0040
Description	HCT116-SFN (-/-) is a cell line with a homozygous knockout of human SFN
Target Gene	SFN
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SFN stratifin [ Homo sapiens ]
Gene Symbol	SFN
Synonyms	YWHAS
Gene Description	stratifin
GeneID	2810
Uni ProtID	P31947
mRNA Refseq	NM_006142.3
Protein Refseq	NP_006133.1
Chromosome Location	1p36.11
Function	phosphoprotein binding; protein binding; protein domain specific binding; protein kinase C inhibitor activity;
Pathway	Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Class I PI3K signaling events mediated by Akt, organism-specific biosystem;
MIM	601290

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

High efficiency

I think the results of this experiment remove the problem of my technique and this transfection reagent still contributes very much.

United States

2023-03-16

Good to use

I must compliment this reagent, the transfection is better than any I have used, I have knocked out some other genes before and none of them were as efficient as this.

United States

2023-01-25

Costly

This reagent may not be as good as the super expensive ones I use overseas, but when you think about its price, this is a real bargain.

United States

2023-01-19

Cheap ahd good results

The price was so cheap, I thought it might not turn out well for such a cheap price. I never wanted to try it before for fear of wasting my time, but now it seems to have turned out quite well.

United States

2023-02-22

Progressing well

The initial validation of the knockout transfection went very well, and then the move to formal experiments was quick and relatively stable.

United States

2020-01-12

Save time and money

This cell line has made my experiments go very quickly, saving time, and it is not expensive, saving my boss a lot of money!

United States

2021-04-11

Successful transfection

The transfections were very successful, and more than once or twice, with reliable results almost every time, completing the current phase of the experiment some time ahead of schedule.

United States

2021-11-21

Nice transfer dye

I chose this brand after hearing a recommendation from an online friend who had used it before, and I came to comment after using it with great success and very good results.

United States

2022-01-31

Stable results

The results of this cell line are very stable, I have done five transfections and the results of three Western blots are almost indistinguishable.

United States

2022-08-05

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Human SFN Knockout Cell Line-HCT116

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

High efficiency

Good to use

Costly

Cheap ahd good results

Progressing well

Save time and money

Successful transfection

Nice transfer dye

Stable results

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