Human SEMA5A(His) HEK293 Cell Lysate

Name: Human SEMA5A(His) HEK293 Cell Lysate
SKU: CLOE-0924
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0924 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0924
Introduction	This cell lysate over-expresses extracellular domain of human SEMA5A (NP_003957.2) (Met 1-Met 968) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	SEMA5A
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A [ Homo sapiens ]
Gene Symbol	SEMA5A
Synonyms	semF; SEMAF
Gene Description	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
GeneID	9037
Uni ProtID	Q13591
mRNA Refseq	NM_003966.2
Protein Refseq	NP_003957.2
Chromosome Location	5p15.2
Function	axon guidance receptor activity;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
MIM	609297

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