Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0778 Host Cell : HEK293
| Cat. No. | CLOE-0778 |
| Description | This cell lysate over-expresses human SEMA4A (NP_071762.2) (Met 1-His 683) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified. |
| Introduction | This cell lysate over-expresses human SEMA4A (NP_071762.2) (Met 1-His 683) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | SEMA4A |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ] |
| Gene Symbol | SEMA4A |
| Synonyms | SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2; |
| GeneID | 64218 |
| Uni ProtID | Q9H3S1 |
| mRNA Refseq | BC020974 |
| Chromosome Location | 1q22 |
| Function | receptor activity; |
| Pathway | Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem; |
| MIM | 607292 |
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