Human SEMA3A HEK293 Cell Lysate

Name: Human SEMA3A HEK293 Cell Lysate
SKU: CLOE-1208
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1208 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1208
Introduction	This cell lysate over-expresses the human SEMA3A (NP_006071.1) (Lys26-Phe546) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	SEMA3A
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ]
Gene Symbol	SEMA3A
Synonyms	SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
Gene Description	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
GeneID	10371
Uni ProtID	Q14563
mRNA Refseq	NM_006080.2
Protein Refseq	NP_006071.1
Chromosome Location	7p12.1
Function	chemorepellent activity; receptor activity;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; CRMPs in Sema3A signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion, organism-specific biosystem; Sema3A PAK dependent Axon repulsion, organism-specific biosystem;
MIM	603961

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