Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0974 Host Cell : HEK293
| Cat. No. | CLOE-0974 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human RSPO3 (Q9BXY4-1) (Met 1-Val 146). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | RSPO3 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | RSPO3 R-spondin 3 [ Homo sapiens ] |
| Gene Symbol | RSPO3 |
| Synonyms | RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis) , thrombospondin, type I, domain containing 2 , THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1; |
| Gene ID | 84870 |
| Uni Prot ID | Q9BXY4 |
| m RNA Refseq | BC022367 |
| Chromosome Location | 6q22.33 |
| Function | frizzled binding; heparin binding; receptor binding; |
| MIM | 610574 |
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