Human RASGRP3 Knockout Cell Line-A549

Name: Human RASGRP3 Knockout Cell Line-A549
SKU: CSC-RT2234
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2234

Target Gene : RASGRP3 Host Cell : A549

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2234
Description	This cell is a stable cell line with a homozygous knockout of human RASGRP3 using CRISPR/Cas9.
Target Gene	RASGRP3
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nirtogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated) [ Homo sapiens ]
Gene Symbol	RASGRP3
Synonyms	GRP3
Gene Description	RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Gene ID	25780
Uni Prot ID	Q8IV61
m RNA Refseq	NM_001139488.1
Protein Refseq	NP_001132960.1
Chromosome Location	2p25.1-p24.1
Pathway	Activation of RAS in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Downstream Signaling Events Of B Cell Receptor (BCR), organism-specific biosystem; Immune System, organism-specific biosystem;
MIM	609531

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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