Human RAP1A adenoviral particles

RAP1A is a member of the Ras superfamily of small GTPases that plays a key role in processes such as cell adhesion, proliferation, and signal transduction. The RAP1A gene is located on human chromosome 1p13.3 and the protein it encodes cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch that regulates integrin-mediated cell-matrix interactions, vesicle trafficking, and the MAP kinase pathway. RAP1A is highly conserved across species and shares structural homology with other Ras-related proteins, with a C-terminal prenylation motif essential for membrane localization. Dysregulation of RAP1A has been implicated in cancer, immune disorders, and cardiovascular disease, making it a key target for mechanistic studies and therapeutic development.

Human RAP1A Adenoviral Particles are recombinant adenoviral vectors designed to efficiently deliver the RAP1A gene to mammalian cells. These particles exploit the natural infectivity of adenoviruses to enable stable transgene expression even in cells that are difficult to transfect, such as primary or non-dividing cells. To ensure safety, we have modified the adenoviral backbone by removing essential viral replication genes (E1/E3 deletion) and replacing them with the RAP1A coding sequence under the control of a strong promoter such as CMV or EF1α. These particles are ideal for gain-of-function studies, allowing researchers to investigate the role of RAP1A in signaling pathways, cell migration, or cancer metastasis.