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Human PPP1CC adenoviral particles

Human PPP1CC adenoviral particles

Cat.No. :  AD00234Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Cat. No. AD00234Z
Target Gene PPP1CC
Species Human
Product Type Adenoviral particle
Insert PPP1CC
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
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The PPP1CC gene encodes protein phosphatase 1 catalytic subunit γ (PP1γ), which belongs to the serine/threonine phosphatase family. PP1 plays a key role in a variety of cellular processes, including cell cycle progression, glycogen metabolism, apoptosis, and neuronal signaling. PPP1CC isoforms, particularly PP1γ, are highly conserved and widely expressed in tissues, especially in the testis and brain. It modulates key signaling pathways by dephosphorylating target proteins, thereby regulating their activity. PPP1CC dysregulation has been implicated in diseases such as cancer, neurodegenerative diseases, and male infertility. Human PPP1CC Adenoviral Particles are genetically engineered viral vectors designed to deliver the PPP1CC gene to mammalian cells for functional studies or therapeutic applications. These particles are based on a replication-defective adenovirus, which ensures efficient transduction of a variety of cell types, including dividing and non-dividing cells. This adenoviral system has high transduction efficiency and stable transgene expression, making it an ideal tool for overexpressing PPP1CC in vitro or in vivo. These particles can be used to study the role of PP1γ in cell signaling, disease mechanisms, or potential gene therapy approaches.
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Customer Reviews
Consistent Quality

We’ve ordered multiple batches, and each one has been consistent in titer and performance. No contamination issues—great for long-term experiments.

Canada

02/20/2024

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