Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0610 Host Cell : HEK293
| Cat. No. | CLOE-0610 |
| Description | This cell lysate over-expresses human PLA2G2D (Q9UNK4) (Met1-Cys145) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified. |
| Introduction | This cell lysate over-expresses human PLA2G2D (Q9UNK4) (Met1-Cys145) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | PLA2G2D |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | PLA2G2D phospholipase A2, group IID [ Homo sapiens ] |
| Gene Symbol | PLA2G2D |
| Synonyms | SPLASH; sPLA2S |
| Gene Description | phospholipase A2, group IID |
| GeneID | 26279 |
| Uni ProtID | Q9UNK4 |
| mRNA Refseq | NM_012400.2 |
| Protein Refseq | NP_036532.1 |
| Chromosome Location | 1p36.12 |
| Function | calcium ion binding; phospholipase A2 activity; |
| Pathway | Acyl chain remodelling of PC, organism-specific biosystem; Acyl chain remodelling of PE, organism-specific biosystem; Acyl chain remodelling of PG, organism-specific biosystem; Acyl chain remodelling of PI, organism-specific biosystem; Acyl chain remodelling of PS, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; |
| MIM | 605630 |
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