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Human PDPK1 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0063

Target Gene :   PDPK1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0063
Description HCT116 -PDPK1 (+/-) is a cell line with a heterozygous knockout of human PDPK1
Target Gene PDPK1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name Homo sapiens 3-phosphoinositide dependent protein kinase-1 DNA.
Gene Symbol PDPK1
Synonyms PDK1, PRO0461, MGC20087, MGC35290, PDPK1
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Improving experimental quality

I'm amazed by the consistency of these PDPK1 knockout cell line. They always deliver accurate and reliable results, and have greatly improved the quality of my experiments.

United States

Correct trend

Imagine being able to conduct your experiments with complete confidence in your results. That's exactly what these PDPK1 knockout cell line have done for me!

United States

Experimentation made easier

These PDPK1 knockout cell line are efficient ! They've made my experiments so much easier and more successful, and have greatly improved the accuracy of my results.

United States

Effective

Human PDPK1 Knockout Cell Line-HCT116 reagent is a Experimental saviour! It has saved me so much time and effort in my research. The knockdown efficiency is incredible and the product is very easy to use.

United States

High-quality results

I have tried several gene knockout reagents in the past, but Human PDPK1 Knockout Cell Line-HCT116 is by far the best. It consistently produces high-quality results, and the knockdown efficiency is impressive.

United States

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