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Cat.No. : CSC-RT0063
Host Cell: HCT116 Target Gene: PDPK1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0063 |
| Description | HCT116 -PDPK1 (+/-) is a cell line with a heterozygous knockout of human PDPK1 |
| Target Gene | PDPK1 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I'm amazed by the consistency of these PDPK1 knockout cell line. They always deliver accurate and reliable results, and have greatly improved the quality of my experiments.
United States
03/15/2023
Imagine being able to conduct your experiments with complete confidence in your results. That's exactly what these PDPK1 knockout cell line have done for me!
United States
04/06/2023
These PDPK1 knockout cell line are efficient ! They've made my experiments so much easier and more successful, and have greatly improved the accuracy of my results.
United States
05/05/2023
Human PDPK1 Knockout Cell Line-HCT116 reagent is a Experimental saviour! It has saved me so much time and effort in my research. The knockdown efficiency is incredible and the product is very easy to use.
United States
07/19/2022
I have tried several gene knockout reagents in the past, but Human PDPK1 Knockout Cell Line-HCT116 is by far the best. It consistently produces high-quality results, and the knockdown efficiency is impressive.
United States
11/03/2022
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