Human PDE1B Stable Cell Line-CHO-CNG-sGC

Name: Human PDE1B Stable Cell Line-CHO-CNG-sGC
SKU: CSC-RG1628
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1628

Host Cell : CHO-CNG-sGC Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1628
Description	This cell line is engineered to stably overexpress the human PDE1B in CHO-CNG-sGC cells.
Background	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Target Gene	PDE1B
Gene Species	Homo sapiens (Human)
Host Cell	CHO-CNG-sGC
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-CNG-sGC
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PDE1B phosphodiesterase 1B, calmodulin-dependent [ Homo sapiens ]
Gene Symbol	PDE1B
Synonyms	PDE1B; phosphodiesterase 1B, calmodulin-dependent; PDES1B; calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1B; cam-PDE 1B; 63 kDa Cam-PDE; calmodulin-stimulated phosphodiesterase PDE1B1; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B; PDE1B1;
GeneID	5153
Uni ProtID	Q01064
mRNA Refseq	BC032226
Chromosome Location	12q13
Function	3,5-cyclic-AMP phosphodiesterase activity; calcium- and calmodulin-regulated 3,5-cyclic-GMP phosphodiesterase activity; calmodulin binding; calmodulin-dependent c
Pathway	Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Calmodulin induced events, organism-specific biosystem; Cam-PDE 1 activation, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem;
MIM	171891

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