Cat.No. : CSC-RT0558
Host Cell: HeLa Target Gene: PAM
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0558 |
| Description | A stable cell line with a homozygous knockout of human PAM using CRISPR/Cas9. |
| Target Gene | PAM |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: DMEM supplemented with 10% fetal bovine serum.
It is not required to add the selection antibiotics when culturing the KO cells.
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human PAM Knockout Cell Line-HeLa is an invaluable tool for our research, providing us with consistent and reliable results. We have been using it in our lab for several months, and it has significantly improved our understanding of cell signaling pathways.
The Human PAM Knockout Cell Line-HeLa has been a game-changer for our research. The cells are of high quality and the knockout efficiency is remarkable. The comprehensive documentation provided made it easy for us to incorporate this cell line into our existing experiments.
We were impressed by the level of support we received from the company during and after our purchase. They answered all our questions promptly and provided us with valuable tips for maintaining and using the cell line effectively.
The Human PAM Knockout Cell Line-HeLa has been a versatile tool in our research, allowing us to explore various aspects of cell biology. We have used it in several assays, including western blotting, immunofluorescence, and cell proliferation assays, with excellent results.
We have been using the Human PAM Knockout Cell Line-HeLa in our lab for some time now, and we have found it to be a reliable and consistent resource. The cells are easy to work with, and their knockout efficiency has been a significant advantage in our research. We highly recommend this product to other labs
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