Human NPC1 HEK293 Cell Lysate

Name: Human NPC1 HEK293 Cell Lysate
SKU: CLOE-0028
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0028 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0028
Description	This cell lysate over-expresses human NPC1 (Arg372-Phe622) with His-FLAG tag at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses human NPC1 (Arg372-Phe622) with His-FLAG tag at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	NPC1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	NPC1 Niemann-Pick disease, type C1 [ Homo sapiens ]
Gene Symbol	NPC1
Synonyms	NPC
Gene ID	4864
Uni Prot ID	O15118
m RNA Refseq	NM_000271.4
Protein Refseq	NP_000262.2
Chromosome Location	18q11.2
Function	cholesterol binding; hedgehog receptor activity; protein binding; receptor activity; sterol transporter activity; transmembrane signaling receptor activity;
Pathway	Lysosome, organism-specific biosystem; Lysosome, conserved biosystem;
MIM	607623

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