Human MGAT5 HEK293 Cell Lysate

Name: Human MGAT5 HEK293 Cell Lysate
SKU: CLOE-0899
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0899 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0899
Description	This cell lysate over-expresses human MGAT5 (NP_002401.1) (Leu 189-Leu 741) fused with N-terminal signal peptide and C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses human MGAT5 (NP_002401.1) (Leu 189-Leu 741) fused with N-terminal signal peptide and C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	MGAT5
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase [ Homo sapiens ]
Gene Symbol	MGAT5
Synonyms	GNT-V; GNT-VA
GeneID	4249
Uni ProtID	Q09328
mRNA Refseq	NM_002410.4
Protein Refseq	NP_002401.1
Chromosome Location	2q21.3
Function	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity;
Pathway	Asparagine N-linked glycosylation, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan antennae elongation, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan antennae elongation in the medial/trans-Golgi, organism-specific biosystem; N-glycan biosynthesis, complex type, organism-specific biosystem;
MIM	601774

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