Pages
Products

Human MEA1 Knockout Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0946

Target Gene :   MEA1 Host Cell :   HEK293T

Size :   >1x106 cells/vial Validation :   Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0946
Description A stable cell line with a homozygous knockout of human MEA1 using CRISPR/Cas9.
Target Gene MEA1
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Storage Liquid nitrogen
Shipping Dry ice package
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name MEA1
GeneID 4201
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Robustness and efficiency

I am impressed with the robustness and efficiency of the Human MEA1 Knockout Cell Line-HeLa. It has revolutionized our understanding of glucocorticoid receptor function, providing valuable insights into hormone regulation

Germany

Ease of use and consistent results

The Human MEA1 Knockout Cell Line-HeLa has provided us with a powerful tool to investigate the involvement of MEA1 in cellular differentiation. Its ease of use and consistent results have accelerated our understanding of this process.

United States

Exceptional quality

The exceptional quality and performance of the Human MEA1 Knockout Cell Line-HeLa have greatly facilitated our research on MEA1-related diseases. Its versatility and precision have allowed us to uncover novel therapeutic targets.

United States

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction