Human LigIII Knockdown Cell Line-HeLa

Name: Human LigIII Knockdown Cell Line-HeLa
SKU: CSC-RK0008
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0008

Host Cell : HeLa Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-RK0008
Background	This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Target Gene	LIG3
Abbr	HeLa-HuLigIII Knockdown
Alias	LIG3, DNA ligase 3, LIG2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Morphology	Epithelial
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LIG3 ligase III, DNA, ATP-dependent [ Homo sapiens ]
Gene Symbol	LIG3
Synonyms	LIG3; ligase III, DNA, ATP-dependent; LIG2, ligase II, DNA, ATP dependent; DNA ligase 3; ligase II, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3; LIG2;
Gene ID	3980
Uni Prot ID	P49916
m RNA Refseq	BC009026
Chromosome Location	17q11.2-q12
Function	ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; ligase activity; metal ion binding; nucleotide binding; protein binding; zinc ion binding;
Pathway	BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; DNA Repair, organism-specific biosystem; Resolution of AP sites via the single-nucleotide replacement pathway, organism-specific biosystem;
MIM	600940

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