Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1578 Host Cell : HEK293
| Cat. No. | CLOE-1578 |
| Introduction | This cell lysate over-expresses the human LEFTY2 (NP_003231.2) (Phe 78-Pro 366) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | LEFTY2 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | LEFTY2 left-right determination factor 2 [ Homo sapiens ] |
| Gene Symbol | LEFTY2 |
| Synonyms | LEFTY2; left-right determination factor 2; EBAF, endometrial bleeding associated factor (left right determination, factor A; transforming growth factor beta superfamily) , TGFB4; LEFTA; LEFTYA; transforming growth factor; beta 4 (endometrial bleeding associated factor; LEFTY A); TGF-beta-4; protein lefty-2; protein lefty-A; left-right determination factor A; transforming growth factor beta-4; endometrial bleeding-associated factor; EBAF; TGFB4; MGC46222; |
| GeneID | 7044 |
| Uni ProtID | O00292 |
| mRNA Refseq | BC035718 |
| Chromosome Location | 1q42.1 |
| Function | cytokine activity; growth factor activity; transforming growth factor beta receptor binding; |
| Pathway | Developmental Biology, organism-specific biosystem; Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Regulation of Signaling by NODAL, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Signaling by NODAL, organism-specific biosystem; |
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