Human LCAT HEK293 Cell Lysate

Name: Human LCAT HEK293 Cell Lysate
SKU: CLOE-0487
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0487 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0487
Description	This cell lysate over-expresses his tagged human LCAT (NP_000220.1) (Met1-Glu440). Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses his tagged human LCAT (NP_000220.1) (Met1-Glu440). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	LCAT
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
Gene Symbol	LCAT
Gene Description	lecithin-cholesterol acyltransferase
Gene ID	3931
Uni Prot ID	P04180
m RNA Refseq	NM_000229.1
Protein Refseq	NP_000220.1
Chromosome Location	16q22.1
Function	apolipoprotein A-I binding; phosphatidylcholine-sterol O-acyltransferase activity; phosphatidylcholine-sterol O-acyltransferase activity; phospholipase A2 activity; protein binding;
Pathway	Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; HDL-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem;
MIM	606967

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