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Human KDM5C Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0060

Target Gene :   KDM5C Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0060
Description HCT116 -KDM5C (-/Y) is a cell line with knockout of X-linked XIAP allele in male parental cells
Target Gene KDM5C
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name KDM5C lysine (K)-specific demethylase 5C [ Homo sapiens ]
Gene Symbol KDM5C
Synonyms MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Gene ID 8242
Uni Prot ID P41229
m RNA Refseq NM_001146702.1
Protein Refseq NP_001140174.1
Chromosome Location Xp11.22-p11.21
Function DNA binding; histone demethylase activity (H3-K4 specific); molecular_function; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; zinc ion binding;
MIM 314690
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Dependable

I'm so impressed with the quality of these KDM5C knockout cell line. They're incredibly reliable and effective, and have helped me streamline my experiments.

United States

Improving efficiency

This brand's KDM5C knockout cell line are completely revolutionized the way I conduct my experiments, and have made them much more efficient and effective.

United States

Precise results

I can't recommend these KDM5C knockout cell line enough! They're the best on the market, and have consistently delivered reliable and accurate results.

United States

Successful experiment

Thanks to these cell lines, I've been able to achieve amazing success in my experiments. They're simply fantastic!

United States

Highly efficient and affordable

These KDM5C knockout cell line are a good tool for any researcher. They're incredibly reliable, efficient, and affordable, and have greatly improved the accuracy and consistency of my results.

United States

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